Methods in Molecular Genetics

  • Start

    2022-07-18

  • price

    600,00 euros eur

  • Credit

    3 ECTS

  • time

    2 weeks (90 hours)

  • Language

    English

Field of study
Life Sciences
Application deadline
1 June 2022
Dates
18-07-2022 – 29-07-2022
Course mode
On-site
Course Leader

Jana Radzijevskaja is professor at the Department of Biology at Vytautas Magnus University, Faculty of Natural Sciences and chief researcher at the Centre of Environmental research.

Her research interests include molecular ecology, population genetics, vector-borne diseases and molecular systematics. She has extensive experience in the study of ticks and tick-borne pathogens.  Research directions:  identification and genetic characterization of  the most important vectors of vector-borne diseases; diagnosis and genetic analysis of vector-borne pathogens (tick-borne encephalitis virus; borrelia, anaplasma, rickettsia and bartonella bacteria; babesia parasites; filaroid nematodes) in vectors, wild and domestic animals; investigation of host-parasites relationships;  population genetic studies of various animal species based on nuclear and mitochondrial genome markers.

Prof. dr. Jana Radzijevskaja takes an active part in research projects in Lithuania as well as abroad.

Target group
Bachelor level, Master level and PhD students, researchers and university teachers from areas of biology, bioinformatics, genetics, molecular biology, microbiology, biotechnology, bioengineering, biophysics, health sciences.
Entry requirements
Basic knowledge of genetics.
Short description
This course is a comprehensive overview of key techniques in molecular biology mostly related to DNA analysis. The course covers the techniques of DNA/RNA extraction, gel electrophoresis, PCR and restriction digestion; data analysis using computer software. Upon completion of this course, the trainee would be able to extract genomic DNA/RNA from animal cells, perform agarose gel electrophoresis to analyse the DNA profile; amplify specific fragments of DNA using the polymerase chain reaction (PCR) technique; perform restriction digestion experiments and visualise data obtained. School consists of lectures, practical exercises in laboratories, data analysis and case studies seminars.
Aim
To cover the underlying principles and critical aspects of DNA analysis methodology and interpretation.
Learning outcomes

By the end of the course students will be able to:

  • Understand the fundamentals of DNA analysis with significant hands-on experience;
  • Work and properly interpret DNA analysis results


Programme Structure
Topics:

  • basic genetics knowledge
  • DNA extraction
  • gel electrophoresis
  • polymerase chain reaction
  • restriction digestion
  • data visualisation and interpretation


Fee
600,00 euros